模拟热浪及强降温对Apo E-/-小鼠冠心病影响的实验研究
Effects of Simulated Heat Wave and Strong Cooling on Risk Factors for Coronary Heart Disease of Apo E Knockout Mice
中文摘要：
模拟一次典型天气过程，对比过程中ApoE-/-小鼠体重、肛温及HSP60、SOD、TNF、sICAM-1、HIF-1α、ET-1、NO的变化，探讨冷热刺激对小鼠的影响，为进一步探讨对心脑血管疾病的影响奠定基础。通过分析南京市实际气象要素资料，选取一次典型天气过程，并利用气象环境模拟箱模拟，将Apo E-/-小鼠分为实验组和对照组，每组6只，测量实验过程中各组小鼠体重、肛温，采用ELISA法测定热浪后小鼠HSP60、SOD、TNF、sICAM-1、HIF-1-、ET-1、NO变化情况。结果显示，小鼠体重不存在统计学差异（P＞0.05），但在模拟实验过程结束后有所下降（P＞0.05）。肛温热浪组与对照组相比有所升高（P＞0.05），强降温组则又下降。HSP60、SOD、TNF、sICAM-1、HIF-1-表达水平上热浪组均高于对照组，与热浪组相比强降温组有所下降且具有统计学差异（P＜0.05）。强降温将导致血管收缩。结果表明，热浪过程会加重动脉粥样硬化疾病的发生发展，强降温过程可能会极大地增加冠心病等心血管疾病的发病和死亡风险。
中文关键词：
热浪，强降温，Apo E-/-小鼠，动脉粥样硬化，心脑血管疾病
KeyWords：
heat wave, strong cooling, Apo E Knockout Mice, atherosclerosis, cardiovascular disease
Abstract：
Objective: Simulate a typical weather process and compare the levels of Apo E Knockout Mice’ HSP60, SOD, TNF,sICAM-1, HIF-1α, ET-1, NO, body weight and rectal temperature to study the effects of hot and cold stimulation in mice. In order
to further investigate the impact of heat wave on cardiovascular disease, eighteen Apo E Knockout Mice were divided into the heat wave group, strong cooling group and control group. The body weight and rectal temperature of each mouse were measured every day. By use of meteorological environment simulation box, the process of heat wave was stimulated, determining HSP60, SOD, TNF,sICAM-1, HIF-1α, ET-1, NO of Apo E Knockout Mice, measured by enzyme linked immunosorbent assay (ELISA). The result is as follows, HSP60, SOD, TNF, sICAM-1, HIF-1α and rectal temperature of Apo E Knockout Mice in group of heat wave were higher than that in strong cooling and control group. Body weight showed no significant difference but risk increased after the simulation process in strong cooling group. Strong cooling will cause vasoconstriction. Conclusion: Thermal stimulation may aggravate atherosclerosis disease development. The process of Strong Cooling may greatly increase the risk of coronary heart disease.
to further investigate the impact of heat wave on cardiovascular disease, eighteen Apo E Knockout Mice were divided into the heat wave group, strong cooling group and control group. The body weight and rectal temperature of each mouse were measured every day. By use of meteorological environment simulation box, the process of heat wave was stimulated, determining HSP60, SOD, TNF,sICAM-1, HIF-1α, ET-1, NO of Apo E Knockout Mice, measured by enzyme linked immunosorbent assay (ELISA). The result is as follows, HSP60, SOD, TNF, sICAM-1, HIF-1α and rectal temperature of Apo E Knockout Mice in group of heat wave were higher than that in strong cooling and control group. Body weight showed no significant difference but risk increased after the simulation process in strong cooling group. Strong cooling will cause vasoconstriction. Conclusion: Thermal stimulation may aggravate atherosclerosis disease development. The process of Strong Cooling may greatly increase the risk of coronary heart disease.